Cool! Is it known how to sequence the haploid cell? Can you get a haploid cell to divide so you can feed it into PCR or something? (I’m a layperson w.r.t. biology.) I just recently had an idea about sequencing sperm by looking at their meiotic cousins and would be interested in talking in general about this topic; email at gmail, address tsvibtcontact.
https://tsvibt.blogspot.com/2022/06/non-destructively-sequencing-gametes-by.html
Yes, you can let them divide and then use the usual (destructive) sequencing.
And also yes, sequencing meiotic cousins of sperm (or polar bodies, in the case of eggs) is a promising concept. Unfortunately primary spermatocytes won’t do meiosis if you isolate them; the environment of the seminiferous tubules is very important. So you would have to be able to track them within the tubules in an organ culture system.
Polar body biopsies for eggs are much more feasible (I have done them, although I haven’t sequenced the polar bodies). Unfortunately the efficacy of selection is limited by the number of eggs.
Cool! Is it known how to sequence the haploid cell? Can you get a haploid cell to divide so you can feed it into PCR or something? (I’m a layperson w.r.t. biology.) I just recently had an idea about sequencing sperm by looking at their meiotic cousins and would be interested in talking in general about this topic; email at gmail, address tsvibtcontact. https://tsvibt.blogspot.com/2022/06/non-destructively-sequencing-gametes-by.html
Oh I see, the haploid cells are, like, independently viable and divide and stuff.
Yes, you can let them divide and then use the usual (destructive) sequencing.
And also yes, sequencing meiotic cousins of sperm (or polar bodies, in the case of eggs) is a promising concept. Unfortunately primary spermatocytes won’t do meiosis if you isolate them; the environment of the seminiferous tubules is very important. So you would have to be able to track them within the tubules in an organ culture system.
Polar body biopsies for eggs are much more feasible (I have done them, although I haven’t sequenced the polar bodies). Unfortunately the efficacy of selection is limited by the number of eggs.