Yes, you can let them divide and then use the usual (destructive) sequencing.
And also yes, sequencing meiotic cousins of sperm (or polar bodies, in the case of eggs) is a promising concept. Unfortunately primary spermatocytes won’t do meiosis if you isolate them; the environment of the seminiferous tubules is very important. So you would have to be able to track them within the tubules in an organ culture system.
Polar body biopsies for eggs are much more feasible (I have done them, although I haven’t sequenced the polar bodies). Unfortunately the efficacy of selection is limited by the number of eggs.
Yes, you can let them divide and then use the usual (destructive) sequencing.
And also yes, sequencing meiotic cousins of sperm (or polar bodies, in the case of eggs) is a promising concept. Unfortunately primary spermatocytes won’t do meiosis if you isolate them; the environment of the seminiferous tubules is very important. So you would have to be able to track them within the tubules in an organ culture system.
Polar body biopsies for eggs are much more feasible (I have done them, although I haven’t sequenced the polar bodies). Unfortunately the efficacy of selection is limited by the number of eggs.