Adding cryoprotectants will change osmotic pressure and the cell will dessicate, and the open/closedstate will be lost.
Now you know why I’m so keen on the idea of figuring out a way to get something like trehalose into the cell. Neurons tend to lose water rather than import cryoprotectants because of their myelination. Trehalose protects against dessication by cushioning proteins from hitting each other. Other kinds of solute that can get past the membrane could balance out the osmotic pressure (that’s kind of the point of penetrating cryoprotectants) just as well, but I like trehalose because of its low toxicity.
Now you know why I’m so keen on the idea of figuring out a way to get something like trehalose into the cell. Neurons tend to lose water rather than import cryoprotectants because of their myelination. Trehalose protects against dessication by cushioning proteins from hitting each other. Other kinds of solute that can get past the membrane could balance out the osmotic pressure (that’s kind of the point of penetrating cryoprotectants) just as well, but I like trehalose because of its low toxicity.