Sox2-17 enhanced episomal OKS MEF reprogramming by a striking 150 times, giving rise to high-quality miPSCs that could generate all-iPSC mice with up to 77% efficiency
For human cells, up to 9% (if I’m understanding this part correctly).
SOX2-17 gave rise to 56 times more TRA1-60+ colonies compared with WT-SOX2: 8.9% versus 0.16% overall reprogramming efficiency.
So seems like you can do wildly different depending on the setting (mice, humans, bovine, etc), and I don’t know what the Retro folks were doing, but does make their result less impressive.
Thinking through it more, Sox2-17 (they changed 17 amino acids from Sox2 gene) was your linked paper’s result, and Retro’s was a modified version of factors Sox AND KLF. Would be cool if these two results are complementary.
I don’t think that’s right, see https://www.cell.com/cell-stem-cell/fulltext/S1934-5909(23)00402-2
You’re right! Thanks
For Mice, up to 77%
For human cells, up to 9% (if I’m understanding this part correctly).
So seems like you can do wildly different depending on the setting (mice, humans, bovine, etc), and I don’t know what the Retro folks were doing, but does make their result less impressive.
(Still impressive and interesting of course, just not literally SOTA.)
Thinking through it more, Sox2-17 (they changed 17 amino acids from Sox2 gene) was your linked paper’s result, and Retro’s was a modified version of factors Sox AND KLF. Would be cool if these two results are complementary.