As I understand it, the major advantage is that doing the cross-linking first (e.g. w glutaraldehyde) saves you time and maintains blood vessels so that traditional cryoprotectants can diffuse more widely across brain tissue. It also may allow easier validation of the cryopreservation protocol, because you don’t have as many dehydration issues.
As I understand it, the major advantage is that doing the cross-linking first (e.g. w glutaraldehyde) saves you time and maintains blood vessels so that traditional cryoprotectants can diffuse more widely across brain tissue. It also may allow easier validation of the cryopreservation protocol, because you don’t have as many dehydration issues.