Purification isn’t necessary if you buy already purified protein; in my case it was just cheaper to get it in bulk and filter it myself.
Removing the his-tag reduces the low-ish risk of it interfering with the immune response, but not doing so doesn’t strike me as dangerous, it’s just a dangling chain of histidine after all (and biology doesn’t quite work like Unsong, luckily).
As for using peptide vaccines as a booster, I’m mildly optimistic given the evidence. Boosting vector vaccines with mRNA seems stronger than vice-versa, but it’s still better than only having n-1 vaccines. I could see the same being true for peptide vaccines.
FWIW, my first official and fourth overall vaccination pretty much knocked me out for two days, so perhaps my experiment wasn’t entirely without effect.
Thanks for taking the time to answer. When you say purified protein, what’s the standard for “pure enough”? I see some listings that say things like “>95% by SDS-PAGE”.
Purification isn’t necessary if you buy already purified protein; in my case it was just cheaper to get it in bulk and filter it myself.
Removing the his-tag reduces the low-ish risk of it interfering with the immune response, but not doing so doesn’t strike me as dangerous, it’s just a dangling chain of histidine after all (and biology doesn’t quite work like Unsong, luckily).
As for using peptide vaccines as a booster, I’m mildly optimistic given the evidence. Boosting vector vaccines with mRNA seems stronger than vice-versa, but it’s still better than only having n-1 vaccines. I could see the same being true for peptide vaccines.
FWIW, my first official and fourth overall vaccination pretty much knocked me out for two days, so perhaps my experiment wasn’t entirely without effect.
Thanks for taking the time to answer. When you say purified protein, what’s the standard for “pure enough”? I see some listings that say things like “>95% by SDS-PAGE”.