Is there any chance your sequencing had greater than 4x coverage?
I don’t know. How do I find out?
I think the VCF would tell you if you had it. Another possibility would be using a lower quality threshold for calling SNPs, but that seems unlikely.
I don’t know. How do I find out?
I think the VCF would tell you if you had it. Another possibility would be using a lower quality threshold for calling SNPs, but that seems unlikely.