I have not made the specific radvac vaccine, but have read the prep. I’ve done all of those steps for other projects, can confirm, extremely simple and straightforward.
I have had an open offer to my friends to go in halfsies on materials if anyone wants it, but no takers thus far.
As far as QC goes, ‘trust the vendor to mail you the correct peptides’ is the easy route, screwups in that industry are rare and shocking. If you really want to check, raman or IR spectroscopy is probably the way to go—I’ve only ever used ‘the spectrum looks like this, which matches the spectrum in the authoritative source’, I’ve never learned anything about actually reading them. So I couldn’t do that myself...an ELISA assay or something (easy to do if you have it, but idk if one is readily available for those peptides) would work too (take sample of peptide, apply elisa test, if pass, it’s the peptide)
No real reason not to trust the adjuvant or DI water suppliers either.
The actual final product is a kind of crude mixture, and it’s just going up your nose, so you would need to deviate from the procedure pretty severely (like, adding toxic ingredients) in order to really hurt yourself by doing it wrong. It’s not like a drug where microgram differences in dose are the difference between ineffective, effective, and lethal
I have not made the specific radvac vaccine, but have read the prep. I’ve done all of those steps for other projects, can confirm, extremely simple and straightforward.
I have had an open offer to my friends to go in halfsies on materials if anyone wants it, but no takers thus far.
As far as QC goes, ‘trust the vendor to mail you the correct peptides’ is the easy route, screwups in that industry are rare and shocking. If you really want to check, raman or IR spectroscopy is probably the way to go—I’ve only ever used ‘the spectrum looks like this, which matches the spectrum in the authoritative source’, I’ve never learned anything about actually reading them. So I couldn’t do that myself...an ELISA assay or something (easy to do if you have it, but idk if one is readily available for those peptides) would work too (take sample of peptide, apply elisa test, if pass, it’s the peptide)
No real reason not to trust the adjuvant or DI water suppliers either.
The actual final product is a kind of crude mixture, and it’s just going up your nose, so you would need to deviate from the procedure pretty severely (like, adding toxic ingredients) in order to really hurt yourself by doing it wrong. It’s not like a drug where microgram differences in dose are the difference between ineffective, effective, and lethal