“FRET is a non-radiative transfer of energy from an excited donor fluorophore molecule to a nearby acceptor fluorophore molecule...When the biomolecule of interest is present, it can cause a change in the distance between the donor and acceptor, leading to a change in the efficiency of FRET and a corresponding change in the fluorescence intensity of the acceptor. This change in fluorescence can be used to detect and quantify the biomolecule of interest.”
advantages:
real-time
non-destructive
sensitive to very low concentrations (picomolar and nanomolar)
highly specific because it detects conformational changes in biological molecules
this article is from a not-great journal and the author clearly does not have English as a first language… at some point i will need a more reputable source, this was from googling FRET quickly
https://www.astralcodexten.com/p/the-case-against-proposition-36 Clara Collier gives the narrow, evidence-based case that shorter jail sentences didn’t cause California’s property crime wave or drug overdose death epidemic, and longer jail sentences won’t fix those problems
I’m pretty convinced but I don’t follow this topic in great detail
metastatic malignant peripheral nerve sheath tumor is pretty bad—median survival is only 8 months after metastases are detected. but one M.O. that seems to help in several case studies is “sequence the tumor, find a mutation, use a drug that’s approved for other cancer types with the same mutation.”
PD-L1 overexpression? use a PD-1 inhibitor! checkpoint immunotherapy stays winning.
chemo is...not great but better than nothing. some partial responses, no complete responses, survival extended by maybe a few months. mostly it seems best to have doxorubicin in the mix.
mech-interp seems like straightforwardly real and good work from a variety of perspectives on AI. helps with many risk scenarios including some x-risk scenarios; helps make the technology stronger & more reliable, which is good for the industry in the long run.
links 10/30/2024: https://roamresearch.com/#/app/srcpublic/page/10-30-2024
https://pmc.ncbi.nlm.nih.gov/articles/PMC10136898/ FRET is a biosensor modality.
“FRET is a non-radiative transfer of energy from an excited donor fluorophore molecule to a nearby acceptor fluorophore molecule...When the biomolecule of interest is present, it can cause a change in the distance between the donor and acceptor, leading to a change in the efficiency of FRET and a corresponding change in the fluorescence intensity of the acceptor. This change in fluorescence can be used to detect and quantify the biomolecule of interest.”
advantages:
real-time
non-destructive
sensitive to very low concentrations (picomolar and nanomolar)
highly specific because it detects conformational changes in biological molecules
this article is from a not-great journal and the author clearly does not have English as a first language… at some point i will need a more reputable source, this was from googling FRET quickly
https://www.astralcodexten.com/p/the-case-against-proposition-36 Clara Collier gives the narrow, evidence-based case that shorter jail sentences didn’t cause California’s property crime wave or drug overdose death epidemic, and longer jail sentences won’t fix those problems
I’m pretty convinced but I don’t follow this topic in great detail
metastatic malignant peripheral nerve sheath tumor is pretty bad—median survival is only 8 months after metastases are detected. but one M.O. that seems to help in several case studies is “sequence the tumor, find a mutation, use a drug that’s approved for other cancer types with the same mutation.”
PD-L1 overexpression? use a PD-1 inhibitor! checkpoint immunotherapy stays winning.
https://www.spandidos-publications.com/10.3892/ol.2024.14556 sintilimab
https://aacrjournals.org/cancerimmunolres/article/7/9/1396/470072/PD-1-Inhibition-Achieves-a-Complete-Metabolic pembrolizumab
https://scholars.uthscsa.edu/en/publications/pembrolizumab-achieves-a-complete-response-in-an-nf-1-mutated-pd- pembrolizumab
https://ascopubs.org/doi/abs/10.1200/PO.18.00375 nivolumab
BRAF V600E mutation? try a BRAF inhibitor!
https://jnccn.org/view/journals/jnccn/11/12/article-p1466.xml vemurafenib
other Raf stuff: maybe sorafenib?
https://www.tandfonline.com/doi/abs/10.4161/cbt.7.6.5932
shit that doesn’t work:
sirolimus https://onlinelibrary.wiley.com/doi/full/10.1155/2020/5784876
chemo is...not great but better than nothing. some partial responses, no complete responses, survival extended by maybe a few months. mostly it seems best to have doxorubicin in the mix.
https://onlinelibrary.wiley.com/doi/full/10.1155/2017/8685638
https://ascopubs.org/doi/abs/10.1200/JCO.2024.42.16_suppl.11583
https://www.sciencedirect.com/science/article/pii/S0923753419377907
https://ascopubs.org/doi/abs/10.1200/jco.2010.28.15_suppl.e20512
https://onlinelibrary.wiley.com/doi/full/10.1155/2011/705345 ok here’s a complete response to chemo + surgery. it can ever happen.
https://ar.iiarjournals.org/content/40/3/1619.short case of long-term survival after keeping chemotherapy going a *really long time* at gradually decreasing dose and widening inter-treatment interval.
https://onlinelibrary.wiley.com/doi/abs/10.1002/ijc.33201 pazopanib, an angiogenesis inhibitor, similarly has a low response rate but can extend survival a bit
https://proof-scaling-meeting.vercel.app/ formal verification conference
https://chalmermagne.substack.com/p/death-by-a-thousand-roundtables what it’s actually like to work in UK policy. sounds dismal.
https://www.972mag.com/lavender-ai-israeli-army-gaza/ AI bombing. critical perspective on Israel.
https://goingon.org/ a timeline-based, “citizen journalism” news site.
https://statistics.berkeley.edu/about/news/steinhardt-announces-co-founding-transluce-non-profit-ai-research-lab AI interpretability nonprofit, Jacob Steinhardt
mech-interp seems like straightforwardly real and good work from a variety of perspectives on AI. helps with many risk scenarios including some x-risk scenarios; helps make the technology stronger & more reliable, which is good for the industry in the long run.
https://blog.benjaminreinhardt.com/young-people-technical-training this is straightforwardly true, yes, you should learn technical stuff.
https://www.washingtonpost.com/opinions/2024/10/28/jeff-bezos-washington-post-trust/ Jeff Bezos on why the Washington Post isn’t endorsing a Presidential candidate. this is a solidly written persuasive essay; it seemed legit to me, but I could be persuaded otherwise.