You are in good company in thinking that clearing and embedding the tissue in a hydrogel is the best approach. Others with expertise in the area have suggested the same thing to me. I’m just not convinced, so I think that more research is required to tell whether that is the best approach.
ETA: Sorry, just saw your edit. Interesting thoughts on the interaction between preservation and reconstruction. Your perspective and goals make sense to me, although it is not exactly what we are pursuing at Oregon Brain Preservation. We are agnostic as to the potential method of revival and expect this to be relatively further away in the future, if it ever becomes possible.
Yes, more research definitely seems like the best answer to me too.
I’m hopeful that some of these open questions about the relative efficacy of various techniques, and about how to image and then process the data, will be resolved before I’m on my deathbed and need to make a final will / living will.
Thanks for the clarification and your thoughts. In my view, the question is to what extent the polymer gel embedding is helpful from the perspective of maintaining morphomolecular structure, so that it is worth the trade-off of removing the lipids, which could potentially also have information content. https://brainpreservation.github.io/Biomolecules#how-lipid-biomolecules-in-cell-membranes-could-affect-ion-flow
You are in good company in thinking that clearing and embedding the tissue in a hydrogel is the best approach. Others with expertise in the area have suggested the same thing to me. I’m just not convinced, so I think that more research is required to tell whether that is the best approach.
ETA: Sorry, just saw your edit. Interesting thoughts on the interaction between preservation and reconstruction. Your perspective and goals make sense to me, although it is not exactly what we are pursuing at Oregon Brain Preservation. We are agnostic as to the potential method of revival and expect this to be relatively further away in the future, if it ever becomes possible.
Yes, more research definitely seems like the best answer to me too.
I’m hopeful that some of these open questions about the relative efficacy of various techniques, and about how to image and then process the data, will be resolved before I’m on my deathbed and need to make a final will / living will.